Computational H&E staining, combined with 3D imaging, reveals the full structure of tertiary lymphoid structures (TLS) in NSCLC, overcoming 2D sampling bias and enabling the accurate quantification of volume, maturity, and spatial context.
Computational H&E staining combined with non-destructive 3D imaging reveals the true complexity of tertiary lymphoid structures (TLS) in NSCLC. Unlike thin 2D sections that risk misclassification, Alpenglow’s 3Di platform captures entire TLS morphology, volume, and cellular composition, delivering accurate insights into immune architecture and tumor context.
3D segmentation of prostate glands using synthetic CK8 immunofluorescence derived from fluorescent H&E analogues. By combining image-translation models with traditional computer-vision methods, researchers achieved whole-biopsy 3D gland segmentation without manual labeling.
High-resolution 3D imaging of a cleared rabbit retina stained with TO-PRO-3 and eosin, preserving architecture across retinal, choroid, and scleral layers.
3D imaging of prostate organoids stained with TO-PRO-3 and eosin reveals spatial heterogeneity, cellular interactions, and microenvironmental detail.
3D imaging of prostate organoids with LUMI shows ROI selection, multi-well scanning, and high-resolution analysis using TO-PRO-3 and eosin staining.
Whole mouse brain imaged in 3D with TO-PRO-3 and eosin. Scout and Zoom workflows capture glioblastoma ROI, segmentation, and high-resolution morphology.
Scout 3D imaging of a human DRG sample from AnaBios with computational H&E staining, ToPro-3 for nuclei, and eosin for protein structures.
Zoomed 3D imaging of human DRG sample from AnaBios with computational H&E staining, ToPro-3 for nuclei, and eosin for protein structures.
A 3D fly-through of liver tissue stained with computational hematoxylin and eosin, showcasing structures beyond traditional 2D pathology.