Section based confocal imaging can approximate 3D, but it can introduce sampling gaps, distortion, and registration artifacts. Light sheet microscopy is preferred when you need intact tissue context, whole tissue imaging, and quantitative 3D measurements across depth.

Alpenglow’s 3Di™ Hybrid Open-Top Light-Sheet Microscope merges whole-sample speed with sub-micrometer detail in a single system. Designed for cleared tissues, it enables seamless switching between fast Scout mode and high-resolution Zoom mode. With an open-top design, multi-immersion optics, and AI-ready 3D data outputs, the 3Di HOTLS delivers true volumetric imaging at scale, revealing complete tissue architecture without physical sectioning.

Skin is a 3D organ with complex nerves, structures, and patchy inflammation. 3D tissue imaging reveals intact architecture and spatial biology that 2D slides miss. From innervation in atopic dermatitis to vascular mapping in keloids and melanoma, whole-biopsy imaging with AI-powered analysis delivers reproducible, quantitative insights for dermatology research and clinical translation.

3D tissue imaging reveals what 2D slides often miss, intact tissue architecture, rare features, and the true complexity of the microenvironment. By combining high-resolution, non-destructive 3D histology with AI-powered analysis, researchers gain deeper insights in digital pathology, spatial profiling, and quantitative tissue analysis that accelerate discovery and development.