Use cases

3D tissue imaging use cases across spatial biology

Explore how 3D tissue imaging, digital pathology, AI-powered analysis, spatial profiling, and quantitative tissue analysis can support research programs across intact tissue samples and volumetric datasets.

Whole tissue imaging 3D histology AI-powered analysis Spatial profiling

Use case library

Explore 3D imaging across intact tissue applications

Review application examples covering dermatology, immuno-oncology, tertiary lymphoid structures, 2D versus 3D tissue analysis, and ground-truth data for spatial biology research.

3D atopic dermatitis biopsy showing nerves, immune cells, and microvasculature
Dermatology Use case

3D imaging of atopic dermatitis

See how full-volume 3D imaging of skin biopsies reveals immune–epithelial interactions, nerve fibers, and microvascular changes. The use case demonstrates how intact-tissue analysis can quantify inflammatory patterns and spatial changes associated with treatment.

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3D lymphocyte distribution and immune-exclusion analysis in tumor tissue
Immuno-oncology Use case

Decoding immune exclusion in 3D

Explore how 3D tissue imaging and AI-powered analysis map lymphocyte distribution, exclusion zones, and tumor microenvironment architecture across intact tumor samples. The study supports investigation of spatial patterns associated with differential treatment response.

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Comparison of 2D histology and 3D imaging of the same tissue specimen
Why 3D White paper

2D versus 3D tissue analysis

Compare conventional 2D histology with 3D imaging of the same specimen. The use case shows how isolated sections can undercount structures, miss rare features, or incompletely represent tissue architecture, while whole-tissue datasets provide a more complete spatial reference.

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Ground-truth 3D tissue data for immuno-oncology research
Immuno-oncology PDF

Ground-truth 3D data in immuno-oncology

Explore how whole-tissue 3D imaging, Aurora workflows, and quantitative analysis can support immuno-oncology research through full-volume tissue context and image-linked spatial measurements.

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Next step

Want to discuss a 3D imaging use case for your tissue samples?

Connect with Alpenglow Biosciences to discuss sample type, imaging goals, spatial profiling, AI-powered analysis, and quantitative tissue analysis for your research program.