Alpenglow publications
Research from Alpenglow Biosciences and collaborators.
Explore publications connected to 3D tissue imaging, whole tissue imaging, digital pathology, spatial profiling, volumetric datasets, and quantitative tissue analysis.
Selected publications
Foundational technology and translational research
Explore publications connected to open-top light-sheet microscopy, nondestructive 3D pathology, computational analysis, and clinical and translational applications.
Foundational publications on open-top light-sheet microscopy and 3D pathology
Research covering tissue processing, hybrid and multi-immersion open-top light-sheet microscopy, and the foundations of nondestructive volumetric pathology.
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Assessing the effects of a 3D pathology tissue-processing workflow on downstream molecular analyses
Baraznenok, E., et al. bioRxiv preprint, 2026.
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A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues
Glaser, A. K., et al. Nature Methods 19, 613–619, 2022.
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Harnessing non-destructive 3D pathology
Liu, J. T. C., et al. Nature Biomedical Engineering 5, 203–218, 2021.
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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues
Glaser, A. K., et al. Nature Communications 10, 2781, 2019.
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Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens
Glaser, A. K., et al. Nature Biomedical Engineering 1, 0084, 2017.
Clinical and translational studies using 3D pathology and OTLS
Studies applying volumetric pathology, computational analysis, deep learning, tissue microdissection, and open-top light-sheet microscopy to translational and pathology research.
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3D pathology-guided microdissection
Hsieh, H.-C., et al. bioRxiv preprint, 2025.
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Deep-learning triage of 3D pathology datasets for comprehensive and efficient pathologist assessments
Gao, G., et al. bioRxiv preprint, 2025.
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Analysis of 3D pathology samples using weakly supervised AI
Song, A. H., et al. Cell 187, 2502–2520.e17, 2024.
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Ablating Lgr5-expressing prostatic stromal cells activates the ERK-mediated mechanosensory signaling and disrupts prostate tissue homeostasis
Cell Reports, 2022.
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Prostate Cancer Risk Stratification via Nondestructive 3D Pathology with Deep Learning–Assisted Gland Analysis
Xie, W., et al. Cancer Research 82, 334–345, 2022.
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Diagnosing 12 prostate needle cores within an hour of biopsy via open-top light-sheet microscopy
Journal of Biomedical Optics 25, 126502, 2020.
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Open-Top Light-Sheet Microscopy Image Atlas of Prostate Core Needle Biopsies
Reder, N. P., et al. Archives of Pathology & Laboratory Medicine 143, 1069–1075, 2019.
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Rapid pathology of lumpectomy margins with open-top light-sheet microscopy
Glaser, A. K., et al. Biomedical Optics Express 10, 1257–1272, 2019.
Scientific posters
Selected 3D imaging and spatial biology studies
Explore posters and conference abstracts from Alpenglow Biosciences and collaborators covering immuno-oncology, dermatology, liver disease, 3D image analysis, and HOTLS microscopy.
3D tissue-scale automated segmentation with Alpenglow 3Dai spatial analysis suite
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From publications to projects
Ready to apply Alpenglow’s 3D imaging workflows to your research?
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