Why 3D Tissue Imaging?
Because Biology Isn’t Flat.
Explore intact tissue in full spatial context—no sectioning, no artifacts, and no missing information. Our end-to-end 3D platform delivers deeper insight, faster decisions, and greater confidence in every sample.
Essential Use Cases That Demand 3D Imaging
Convoluted shapes
2D slices distort complex structures like vasculature, neurons, and glands, leading to inaccurate metrics.
Alpenglow quantifies full 3D morphology, capturing true volume, path length, branching angles, and even fractal complexity; precision that 2D can’t approximate.
Use cases
Track neuronal architecture in the enteric and peripheral nervous systems
Map vascular remodeling in dementia, tumors, ischemia, and placental development
Quantify fibrotic changes in liver, kidney, and lung disease with volumetric accuracy
Complex Cellular Distributions
2D histology erases microenvironmental context.
Alpenglow captures full 3D distributions across the tumor microenvironment, fibrotic tissue, and immune infiltrates; revealing spatial context that 2D slices erase.
Use cases
Analyze immune-tumor interactions across the full microenvironment
Visualize amyloid and tau deposits in 3D space
Quantify immune cell types and co-localization in inflamed tissue
Rare Objects Detection
Rare cells and drug targets often go undetected in thin sections.
Use cases
Detect genetically labeled rare cells across entire tissue volumes
Assess drug localization in target regions with full-volume context
Track stem and progenitor cells over time
Identify spatially distinct subclones in patient-derived xenografts
Alpenglow’s slide-free 3D imaging uncovers them—across the full tissue volume.
How 3D Imaging Advances Discovery, Validation, and Diagnostics
Mechanism of Action
Drugs work in 3D tissue, not 2D slides.
Alpenglow shows how therapies engage their targets within the whole tissue architecture, revealing immune cell activation, tissue remodeling, and structural response that flat slices obscure.
Applications:
– Spatial tracking of immune activation in solid tumors
– Neuronal remodeling in neuroinflammatory disease
– Fibrosis progression and regression in lung, liver, kidney
Predictive Capabilities
Alpenglow extracts patterns that drive decision-making:
– Distance between immune cells and tumor nests
– Vessel density shifts before efficacy markers
– Off-target accumulation in neural tissue
These spatial features feed predictive models that improve stratification, optimize dosing, and personalize treatment before clinical trials begin.
Target Confirmation and Biodistribution
In preclinical studies, partial slices miss the full picture of where a drug goes and what it touches.
Alpenglow delivers whole-tissue, high-resolution 3D imaging to map biodistribution across blood vessels, nerves, immune zones, and fibrotic regions.
You don’t need to guess from limited sections. You see the full spatial spread—confirming engagement, detecting off-target toxicity, and validating delivery strategies—before clinical trials begin.
Revolutionizing pathology with 3D Spatial Biology.
Traditional 2D histology flattens complex tissue, losing vital spatial context.
3D imaging captures full tissue architecture, providing deeper insights into disease, cellular interactions, and immune responses.
Our Hybrid Open-Top Light-Sheet (OTLS) microscope enables high-resolution, high-throughput analysis of entire tissue samples or multi-well plates, preserving samples’ 3D structure.
With AI-powered software and image processing, researchers can visualize and quantify complex spatial biology, unlocking insights 2D methods miss—transforming the way we understand and treat disease.