This high-resolution 3D tissue-imaging video features an intact mouse colorectal tumor captured as a whole-tissue block.

B220 marks B cells in green, CD3 marks T cells in red, and nuclei are shown in blue to define overall tissue architecture. Combined, these markers reconstruct immune organization in its native spatial context across the full tumor volume.

This is tissue-scale, ground-truth 3D imaging with single-cell resolution, supporting quantitative analysis of immune organization in complex solid tumors.