Low-resolution 3D imaging with Hybrid Open Top Light Sheet (HOTLS) microscopy of an entire lesional Atopic Dermatitis skin punch biopsy highlights epidermal and dermal innervation.

The tissue was stained with TO-PRO-3 (red, nuclei), PGP9.5 (green, nerves), and CD45 (blue, T cells), enabling comprehensive visualization of neuro-immune interactions and inflammatory changes across the full biopsy.

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Image reproduced with permission from Incyte Corporation.