This dataset presents a whole murine heart imaged in 3D, capturing the complexity and detail of vascular networks across scales. Smooth muscle actin (SMA) stains blood vessels in green, providing a vivid view of vascular architecture, while mural cells are highlighted in purple.

Stains used:

• SMA (Green): Labels blood vessels

• Mural cell stain (Purple): Highlights vessel-associated cells

The video begins with large vessels and gradually zooms into specific regions, moving from broad tissue structures to single-cell resolution. This approach clearly reveals pericytes wrapping around vessels, offering a precise view of microvascular organization.

Captured on the Aurora™ 3Di Hybrid Open Top Light Sheet (HOTLS) microscope, this workflow demonstrates how Scout (low-resolution) and Zoom (high-resolution) imaging can be seamlessly combined to analyze entire organs down to cellular detail. With integration into 3Dm data managementand 3Dai AI-powered analysis, the dataset can be quantified for vessel branching, pericyte distribution, and cellular proximity.

This example showcases the scalability of Alpenglow’s end-to-end 3D spatial biology platform, which can seamlessly transition from tiny organoids to whole organs. Such flexibility drives discoveries in cardiovascular research, vascular biology, and translational studies, where quantitative analysis of complex tissue architecture is essential.