At the frontier of cellular research, three-dimensional fluorescence imaging is redefining how scientists study prostate organoids. Unlike traditional 2D microscopy, which flattens complex structures, 3D imaging preserves the full architecture of these miniature organs, exposing biological subtleties that thin slices overlook.

Stains used:

• TO-PRO-3: Highlights nuclei

• Eosin: Labels cytoplasmic structures (pseudocolored for enhanced detail)

Using the Aurora™ 3Di Hybrid Open Top Light Sheet (HOTLS) microscope, organoids are imaged layer by layer, generating vivid, information-rich datasets. With integration into 3Dm data management and 3Dai AI-powered segmentation, researchers can quantify nuclear organization, cytoplasmic morphology, and spatial heterogeneity across entire organoids.

This volumetric approach provides unmatched clarity into cellular interactions, microenvironmental gradients, and spatial variability, offering transformative insights for prostate cancer modeling, drug testing, and precision medicine.

By moving beyond 2D limitations, 3D digital pathology empowers organoid studies with true biological context.