Scientific resources
Technical Resources
Explore the methods, technologies, and analytical workflows behind 3D tissue imaging and spatial biology.
From preparing intact specimens to acquiring high-quality volumetric data and extracting quantitative biological insights, these resources provide practical technical guidance for each stage of the workflow.
Featured technical white papers
From sample preparation to quantitative insights
Explore three companion technical resources covering tissue clearing, artifact-aware 3D imaging, and scalable AI-driven analysis. Each white paper is paired with a recorded webinar and a technical article.
01 / Sample preparation
Tissue Clearing for 3D Tissue Imaging and Spatial Biology
Compare tissue-clearing approaches, understand their trade-offs, and explore considerations for preparing intact samples for volumetric imaging.
02 / Volumetric imaging
3D Light-Sheet Imaging: How to Avoid Artifacts and Ensure High-Quality Volumetric Data
Review light-sheet imaging configurations, acquisition workflows, common artifacts, and processing steps used to produce reliable 3D datasets.
03 / Quantitative analysis
Quantifying Biology at Scale: Considerations for AI-Driven Image Analysis Pipelines
Explore scalable image-analysis pipelines, segmentation, and quantitative spatial measurements, including applications to nerve–immune cell interactions in human skin.
Looking for a specific topic? You can read each technical article or watch its companion webinar before accessing the downloadable paper.
Laboratory resources
Plan your tissue preparation and staining workflow
Access practical information to support sample preparation, antibody selection, and reproducible 3D tissue imaging. Some detailed protocols are provided upon request so our team can share guidance relevant to your samples and experimental goals.
Detailed methods · Available upon request
Staining and tissue preparation protocols
Alpenglow can share detailed protocols and methodological guidance for selected tissue preparation, clearing, and staining workflows. Let us know your sample type, fixation method, and markers of interest so we can identify the most relevant information.
Antibody selection · Reference list
Antibodies optimized for 3D imaging
Explore antibody options evaluated or optimized for Alpenglow's 3D tissue staining workflows. Use the list as a starting point for discussing tissue-specific marker panels and experimental requirements.
Protocol availability and antibody suitability depend on tissue type, sample preparation, and experimental conditions. Contact our team to discuss the appropriate workflow for your project.
Continue learning
More ways to explore 3D spatial biology
These curated resources complement the technical papers, providing additional presentations and application-focused examples.
Recorded presentations
Webinars and scientific discussions
Browse recorded sessions on imaging technologies, workflows, AI analysis, and applications across tissue research.
Explore all webinars →Applied research
3D imaging use cases
Discover examples of how intact-tissue imaging and quantitative analysis can be applied to biological research questions.
Explore 3D histology applications →