This high-resolution 3D visualization of a lesional atopic dermatitis skin biopsy highlights the spatial relationship between nerves and immune cells.

Using 40X Hybrid Open Top Light Sheet (HOTLS) microscopy, the sample is stained with TO-PRO-3 (blue) to reveal nuclei, PGP 9.5 (white) to trace nerve structures, and CD45 (yellow) to identify lymphocytes.

The imaging captures both the broad architecture of epidermis and dermis innervation and the fine-scale clustering of lymphocytes around specific nerves. By preserving tissue integrity and enabling volumetric analysis, this 3D approach provides insights into immune–neural interactions that are hidden in conventional 2D histology.

The dataset demonstrates the power of whole-tissue, non-destructive imaging to advance dermatological research and deepen the understanding of inflammatory skin diseases (see our AD use case).