This dataset presents mouse skin tissue imaged in 3D using fluorescence labeling. PGP9.5 highlights the broader nerve network, YO-PRO-1 labels nuclei, and Nav1.8-associated signal identifies a subset of sensory neurons.

The volumetric view preserves the continuity of nerve fibers across the imaged tissue depth, revealing large nerve bundles, finer branches, crossings, and terminal structures within dermal and epidermal regions.

With appropriate segmentation, the dataset can support measurement of nerve density, fiber length, branching, orientation, and spatial relationships with surrounding cells and tissue structures. These features are relevant to research on cutaneous innervation, sensory biology, pain, itch, inflammation, and treatment-associated changes in nerve architecture.

The tissue was imaged using the Aurora 3D™ Spatial Biology Solution, including the 3Di™ Hybrid Open-Top Light-Sheet microscope.