This dataset presents mouse skin tissue imaged in 3D, with YO-PRO-1 labeling nuclei and PGP9.5 highlighting cutaneous nerve structures.

The volumetric view reveals larger nerve bundles within the dermis as they divide into progressively finer branches and projections across the imaged tissue volume. Preserving these structures in 3D makes it possible to follow nerve continuity, branching patterns, orientation, and relationships with surrounding cells and tissue compartments.

With appropriate segmentation, the dataset can support measurement of nerve density, fiber length, branching frequency, tortuosity, orientation, and regional variation. These features are relevant to research on cutaneous innervation, sensory biology, neuro-immune interactions, pain, itch, and changes associated with disease or treatment.

The tissue was imaged using the Aurora 3D™ Spatial Biology Solution, including the 3Di™ Hybrid Open-Top Light-Sheet microscope.