How can we better identify rare tissue-resident memory T cells when their location and tissue sampling can determine whether they are detected at all?

Join Cynthia Timmers, Executive Director, Translational Sciences Discovery Lab at Incyte, and the Alpenglow Biosciences team for a live webinar exploring this challenge through complementary 2D and 3D approaches.

Drawing on her pharmaceutical industry and translational research experience, Cynthia will discuss tissue-resident memory T cells in vitiligo, where CD8+ CD103+ CD69+ cells are sparsely distributed and can be difficult to capture using standard histology. Their distribution within patchy disease, their presence at active disease edges, and their relationship with follicular structures make tissue sampling particularly important.

Cynthia will provide an overview of vitiligo biology, treatment, and diagnostic approaches, and share data using H&E, IHC, and multiplex immunofluorescence.

Alexandra Alvarsson, Director of Biology, and Brandy Olin, Project Manager at Alpenglow Biosciences, will then explore how 3D tissue imaging can complement established 2D methods. Using volumetric CD8 imaging and examples of sparsely distributed cells, they will discuss tissue preparation and staining workflows and how examining larger intact tissue volumes can help characterize rare cell populations while preserving their spatial context.

Topics include:
• Tissue resident memory T cells in vitiligo
• Challenges of detecting rare and sparsely distributed cells
• H&E, IHC, multiplex immunofluorescence, and 3D imaging
• The impact of tissue sampling on rare cell detection
• Volumetric imaging of CD8+ cells
• Complementary roles of 2D and 3D approaches

The webinar will conclude with a live discussion and audience Q&A.

September 30, 2026 | 1 PM EDT