Low-resolution 3D imaging with Hybrid Open Top Light Sheet (HOTLS) microscopy of an entire lesional Atopic Dermatitis skin punch biopsy highlights epidermal and dermal innervation alongside immune cell infiltration.

The tissue was stained with TO-PRO-3 (blue, nuclei), PGP9.5 (white, nerves), and CD3 (green, T cells), enabling comprehensive visualization of neuro-immune interactions and inflammatory changes across the full biopsy.

The tissue measured approximately 2.5mm X 2.5mm X 2.5mm.

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Image reproduced with permission from Incyte Corporation.