Whole-Tissue 3D View of Mast Cell–Nerve Crosstalk
Experience an intact, fluorescence-labeled human skin biopsy rendered in true 3D.
Tryptase staining illuminates every mast cell in green, TOPRO-3 marks nuclei in blue, and PGP9.5 traces nerves in red, revealing the native architecture of neuroimmune interactions without physical sectioning.
By retaining full cell morphology and spatial relationships, this dataset delivers the ground truth needed to quantify mast-cell density, map nerve proximity, and explore mechanisms underlying chronic itch, fibrosis, and inflammatory skin disorders.
The video demonstrates how volumetric imaging overcomes slice bias, enabling reproducible, high-content analysis essential for translational dermatology and immuno-oncology research.
