3D Imaging of a Rabbit Retina: High-Resolution Tissue Mapping Across Retinal Layers

This video presents a high-resolution 3D visualization of a cleared rabbit retina, imaged intact using the 3Di light-sheet fluorescence microscope.

The tissue was stained with TO-PRO-3 and eosin, then pseudocolored to highlight structural features: nuclei in magenta and cytoplasm in green.

The fly-through captures the full thickness of the sample—approximately 1 x 1 x 4 mm—revealing the spatial organization of the retina, choroid, and sclera.

By imaging the retina in 3D without slicing, we preserve tissue architecture and spatial relationships across layers, enabling accurate morphological and contextual analysis at cellular resolution.