3D Visualization of TLS in Colorectal Tumor Tissue

Written By Elisa Barbieri

This 3D dataset captures a mouse colorectal tumor stained to highlight key immune populations:

  • CD3 for T cells (purple),

  • B220 for B cells (green),

  • YoPro1 for nuclei (blue).

Within the tumor microenvironment, two tertiary lymphoid structures (TLS) are clearly visible, revealing their complex organization in full spatial context.

TLS are dynamic, three-dimensional immune aggregates that are often difficult to detect in thin sections. Using Alpenglow’s light-sheet imaging and AI-powered 3D analysis, the full volume of tissue was imaged without slicing, enabling segmentation, quantification, and measurement of TLS across the intact tumor.

This dataset exemplifies how 3D spatial profiling uncovers critical features, including TLS size, density, immune cell composition, and spatial relationships, delivering insights essential for immuno-oncology research.

Elisa Barbieri
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